Sporulation of Tricarboxylic Acid Cycle Mutants of Bacillus subtilis

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Sporulation of tricarboxylic acid cycle mutants of Bacillus subtilis.

A mutant of Bacillus subtilis 168 lacking aconitase (EC 4.2.1.3) was found to be blocked at stage 0 or I of sporulation. Although adenosine triphosphate levels, which normally decrease in tricarboxylic acid cycle mutants at the completion of exponential growth, could be maintained at higher levels by feeding metabolizable carbon sources, this did not permit the cells to progress further into th...

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Alcohol-resistant sporulation mutants of Bacillus subtilis.

About 80% of Bacillus subtilis cells form spores when grown in nutrient broth. In medium containing various short-chain aliphatic alcohols, the frequency of sporulation was reduced to 0.5%. Mutants sporulated in the presence of alcohols at a frequency of 30 to 40%. Sporulation in the wild-type cells was sensitive to alcohol at the beginning of sporulation (stage zero). Sensitivity to alcohol in...

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Developmental block in citric acid cycle mutants of Bacillus subtilis.

Mutants deficient in different enzymes of the citric acid cycle can be subdivided into two groups according to the frequency at which they produce heat-resistant spores in nutrient sporulation medium. However, the majority of cells can develop in this medium only to the axial filament stage I of sporulation; aconitase and isocitrate dehydrogenase mutants need the addition of glutamate to reach ...

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Phenotypes of pleiotropic-negative sporulation mutants of Bacillus subtilis.

The phenotypic properties of representatives of the five genetic classes of pleiotropic-negative sporulation mutants have been investigated. Protease production, alkaline and neutral proteases, was curtailed in spoA mutants, but the remainder of mutant classes produced both proteases, albeit at reduced levels. The spoA and spoB mutants plaqued phi2 and phi15 at high efficiency, but the efficien...

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Sporulation of Bacillus thuringiensis without concurrent derepression of the tricarboxylic acid cycle.

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1972

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.109.2.886-894.1972